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. Author manuscript; available in PMC: 2013 Jul 22.
Published in final edited form as: Haematologica. 2006 Aug;91(8):1092–1095.

Figure 1.

Figure 1

Human Fpn mutations affect Fpn localization and hepcidin-induced internalization. A. HEK293T cells were transiently transfected with plasmids containing wild type (WT) Fpn-GFP, FpnA77D-GFP, FpnG80S-GFP or FpnN174I-GFP. Eighteen to 24 h after transfection, localization of Fpn-GFP and response to hepcidin were assessed by epifluorescent microscopy. Cells were incubated with or without 1 μg/mL hepcidin for 4 and 24 h to assess hepcidin response. B. 18–24 h after transfection cells were incubated with or without 1 μg/mL hepcidin for 4 h, and extracts were analyzed by western blot analysis using antibody to GFP and actin as a loading control, as described in the Design and Methods.