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. Author manuscript; available in PMC: 2013 Jul 22.
Published in final edited form as: Nat Immunol. 2012 Aug 26;13(10):947–953. doi: 10.1038/ni.2403

Figure 5. HFD induces LTβR-dependent IL23 which is essential for DIO.

Figure 5

(a) WT (C57BL/6), Ltbr+/−, and Ltbr−/− animals were fed HFD for 10 weeks. At the end of diet, animals were sacrificed and colons were removed and cultured overnight. Supernatants were subjected to ELISA for IL23p19p40 and resulting data was normalized per milligram of colon cultured. (b–d) WT (C57BL/6) mice or Il23a−/− animals were challenged with HFD starting at 9 weeks of age or 9 weeks. (b) Weight as a percentage over starting weight is plotted. (c) Perigonadal fat was removed and weighed at the end of diet; weight of fat is plotted. (d) Fat from (c) is plotted as a percentage of body weight. Weight gain as a percentage of starting weight is plotted. (n=5–9 mice per group; growth curve is reflective of 2 independent experiments and statistics demonstrate differences between HFD groups; Student’s t-test for individual points along growth curves; 1-Way Anova with Bonferonni post-test for dot plots: * P <0.05, **P <0.01)