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. 2013 Jun;3(6):120159. doi: 10.1098/rsob.120159

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© 2013 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 3. — HIF-1α modulates gene expression. (a) Total cellular RNA was isolated from DMOG-treated cells, template cDNAs synthesized and used in a Human Cell Motility PCR Array. Data were analysed using a web-based analysis tool (SABiosciences website). The top 10 hits are displayed here. (b,c) Total cellular RNA was isolated from DMOG-treated or HIF-1α overexpressing cells. Template cDNAs were synthesized and semi-quantitative (RT)-PCR was performed using Arp2 (filled bars) and β-PIX-specific (open bars) primers. Relative expression was calculated using densitometric analysis. The results shown are mean ± s.e.m. from each experimental condition over three separate experiments. Statistical significance was calculated using Student's t-test; *p < 0.05, **p < 0.005.