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Genetic map of the CRISPR_1 region deleted by the pop-in/pop-out approach. (a) Genetic map of the CRISPR_1 region. The deleted region is situated between the regions OUT and TARGET. (b) A scheme representing two stages of recombination events generating the mutant ΔCRISPR_1Δcsx1**Δcas4Δ pyrEF. Two paralogues of csx1 are present in this region. The gene csx1* corresponds to the gene Sil_0392 and csx1** to Sil_0393.
