Table 1.

Analyses of core biophysical parameters of mitochondrial motility by KBD, R₂KR₃ and mutations therein. The definitions and significances of biophysical parameters are described.

biophysical parameter	definition	significance
percentage of motile events	number of motile events recorded	overall motility behaviour
absolute duration of pause between runs	duration of a non-motile event between two runs in the anterograde, retrograde or change of directions	regulation of kinesin or dynein motor activity from inactive to active states
frequency of change in direction	number of times a mitochondrion changes from anterograde to retrograde direction or vice versa	regulation of cross-talk between kinesin and dynein
motility persistency	time interval of steady movement of a mitochondrion in anterograde, retrograde or both directions	regulation of duration of motor activity of kinesin, dynein or both from active to inactive states
mean velocity	mean velocity of all motile events in the anterograde, retrograde or both directions	mean ATPase activity of kinesin, dynein or both from several runs
absolute velocity	velocity of a run (motile) event in the anterograde or retrograde direction	absolute ATPase (motor) activity of kinesin or dynein during a run; measures also net displacement resulting from the cooperation between motors (motor ensemble)