Figure 5.

HR and LR animals exhibit individual differences in the epigenetic regulation of the BDNF promoter 6, associated with a coherent regulation of relevant epigenetic factors. a–d, HR and LR animals were exposed to one (A) or four social defeat sessions (R), or to an empty cage of a Long–Evans rat (N), and were killed 2 h and 30 min later for the measure of histone modifications at the BDNF promoter 6 by chromatin immunoprecipitation (a, b) or mRNA levels for relevant epigenetic factors by real-time PCR (c, d). The number of animals per group (n = 4–9) is shown within the columns, and the data are presented as the mean ± SEM. Data were analyzed with a two-way ANOVA with HR/LR and defeat as independent factors, followed by Scheffé's post hoc test where appropriate. *p < 0.05, **p < 0.01 versus LR-N; and ^††p < 0.01 versus same defeat treatment in LR animals; Scheffé's post hoc test.