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. Author manuscript; available in PMC: 2013 Jul 22.
Published in final edited form as: Methods Mol Biol. 2011;714:301–321. doi: 10.1007/978-1-61779-005-8_19

Figure 3. Validation of the mechanical fractionation method via Western and Northern blot analysis.

Figure 3

A) A schematic representation of the mechanical fractionation method of cellular fractionation. B) Protein from the fractions 1 – 6 were analyzed for ER, cytosol and nuclear proteins by Western blot as described in Fig. 1 A. C) Total RNA (1), RNA extracted from the pellets from centrifugation at 500 × g (2), 15,000 × g (3), 44,000 × g (4), 100,000 × g (5) and that from the supernatant from the 100,000 × g spin were analyzed by Northern blot as described in Fig. 1 B.