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. Author manuscript; available in PMC: 2013 Jul 22.
Published in final edited form as: Methods Mol Biol. 2013;963:303–317. doi: 10.1007/978-1-62703-230-8_18
Route Needle size and volume
Oral (PO) 20G × 38 mm gastric gavage needles (plastic or metal)
Dosing volume should be 10 ml/kg and no more than 5 ml/kg

Intraperitoneal (IP) 26 or 27G needle with 1 ml disposable syringe
Dosing volume should be 10 ml/kg and no more than 5 ml/kg

Subcutaneous (SC) 26 or 27G needle with 1 ml disposable syringe
Dosing volume should not exceed 300 ul

Intravenous (IV) 27G to 30G needle with 1 ml disposable syringe
Dosing volume should not exceed 10 ml/kg

Intramuscular (IM) 27G needle with 1 ml disposable syringe
0.05 ml can be injected into calf muscle