Table 2.
Percentages of lymphocyte populations and their subset composition in pleural fluid assessed in healthy subjects and in patients. Results given as median (interquartile range)
| METS | BPLAE | MPM | Healthy subjects | |
|---|---|---|---|---|
|
Lymphocyte populationsa | ||||
| Total CD3+ T-cells |
80.5 (76–87.5) |
85.2 (81–90.1) |
77 (69–84) |
85 (80–88) |
| CD4+ T-cells |
63.2 (53.8-72) |
63.5 (52.5-72) |
54.2 (44–62.5) |
30.0 (21–32) |
| CD8+ T-cells |
19 (15–25) |
22 (16.4-29) |
25 (19.2-33.5) |
53 (45–59) |
| CD4+/CD8+ ratio |
3.8 (2.6-4.8) |
3.2 (2.2-4.5) |
2.2 (1.5-2.9) |
0.59 (0.48-0.67) |
| Treg (CD4low+CD25bright+)b |
4.4 (3–5.9) |
4.4 (2.4-5.3) |
3.1 |
2.2 (1.6-3.7) |
| NK cells (CD3─CD56+) |
3.5 (2.2-7.5) |
2.8 (2–4) |
10 (3.4-12.5) |
16.0 (12.4-24.1) |
| → % of CD16+NK cells |
30 (20–59) |
18 (1–35) |
26 (14–54) |
6% (3–8) |
|
CD4+T cell subsetsb | ||||
| Naïve |
22.7 (14.7-29) |
25.7 (14.3-37) |
11.2 (3.8-27) |
1.6 (1–2.4) |
| TCM |
47.4 (30–62) |
53.5 (42.9-60) |
40.5 (27.5-51) |
13.1 (9.8-19.6) |
| TEM |
21 (11.4-38.2) |
18.6 (10.2-22) |
19.2 (9.3-32) |
85.1 (77.2-88.9) |
| TTD |
0.6 (0.3-1.4) |
1.3 (0.5-1.7) |
0.8 (0.3-1.6) |
0.7 (0.3-1) |
|
CD8+T cell subsetsb | ||||
| Naïve |
15.2 (7.1-37.3) |
26 (22–43.4) |
11.2 (3.9-29.6) |
1.4 (0.9-2.1) |
| TCM |
22.1 (12–34.7) |
27.4 (17.8-44) |
11.6 (7.2-28.3) |
2.3 (1.6-4.7) |
| TEM |
19.7 (4.3-45.3) |
16.1 (10.7-36) |
18.6 (5.6-49.2) |
84.7 (83.1-87) |
| TTD | 12.7 (5.6-22.3) | 13.7 (8–22.9) | 7.4 (2.4-20.7) | 9.5 (8.5-12.2) |
aAs a percentage of the total lymphocytes (defined as CD45bright, low side scatter).
bAs a percentage of the total CD4+ or CD8+ T-cell population. The total may add up to slightly less or more than 100% because medians of individual subsets are given. the interquartile range for the MPM group could not be safely calculated due to the low number of cases; TCM central-memory cells, TEM effector memory cells, TTD terminally-differentiated effector memory.