Table 1.
Summary of compounds identified using MS, MS/MS, and UV spectra
| Peak # | Rt (min) | MW | [M+H]+ or [M-H]- | +/- Ions | MS/MS fragments | Tentative identification | References |
|---|---|---|---|---|---|---|---|
| 1 |
2.3 |
378 |
377 |
- |
257, 257, 230, 187 |
unknown |
|
| 2 |
3.0 |
360 |
361 |
+ |
259, 247, 86, 58 |
vernodaline |
[18,22,23] |
| 3 |
3.6 |
330 |
329 |
- |
229, 211, 99, 83 |
vernudiflorid |
[24] |
| 4 |
3.8 |
346 |
345 |
- |
273, 209, 197,163, 84, 57 |
unknown |
|
| 5 | 4.7 | 314 | 313 | - | 213, 182, 157, 131, | 12,13- dihydroxyoleic acid | [25,26] |
Five peaks in the chromatograms of CACF (Figure 1B) were analyzed. Retention times (Rt), molecular weight (MW), protonated and deprotonated molecules ([M+H]+ and [M-H]-), +/- ions, MS/MS fragments, as well as their proposed identities are as listed. Among the five peaks, 2, 3 and 5 were identified as vernodaline, vernudiflori and 12,13- dihydroxyol, whereas the identities of minor peaks 1 and 4 remained unknown.