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. 2013 Jul 22;8(7):e69495. doi: 10.1371/journal.pone.0069495

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© 2013 Richard et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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V_HH2-Fc antibody was purified twice using Protein G-based chromatography, separated using standard 12% SDS-PAGE under non-reducing (A) and reducing (B) conditions, electrotransferred to PVDF membranes and probed with a polyclonal anti-human IgG (γ-specific) conjugated to AP. Lane 1, molecular weight standard; Lane 2, 100 ng of V_HH2-Fc antibody. See text for an explanation of the asterisk.