Figure 7. In vivo neutralization of α–Cbtx-induced lethality in mice with different α–Cbtx-specific V_HHs and a V_HH2-Fc antibody.

The lowest antibody:toxin molar ratio (MR) required to protect 100% of mice, or the highest antibody:toxin dose tested if 100% protection could not be obtained, are shown. Mice receiving commercial equine N. kaouthia antivenom (10×w/w) served as a positive control while non-specific V_HH P2 pre-incubated with α–Cbtx served as negative control. With the exception of the post-toxin administration experiments, the toxin and the antibody preparations were pre-incubated for 1 hr at 37°C before i.p. administration to mice.