Table 2. Primers used for the construction of the VHH2-Fc antibody.
Primername | Nucleotidesequences |
PCR mutagenesis primers | |
pMM3 SfaSI For | 5′-CCAAAGTCATGCGCGATCGCCGACAAAACACATACTTG-3′ |
pMM3 SfaSI Rev | 5′-CAAGTATGTGTTTTGTCGGCGATCGCGCATGACTTTGG-3′ |
VHH primers | |
VHH BbsI NcoI For | 5′-TTTTTTGAAGACGCCATGGCCCAGGTAAAGCTGGAGG-3′ |
VHH SfaSI Rev | 5′-TTTTTTGCGATCGCTGAGGAGACGGTGACCTGGGTCC-3′ |
VHH Fc primers | |
VHH Fc BbsI SS For | 5′-TTTGAAGACAAAGGTATGGCTAAAACAAATCTCTTTTTA-3′ |
VHH Fc BbsI HK Rev | 5′-TTTGAAGACAAAAGCTCATTATAATTCATCCTTGTGATG-3′ |