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. 2013 Jul 22;8(7):e69495. doi: 10.1371/journal.pone.0069495

Table 2. Primers used for the construction of the VHH2-Fc antibody.

Primername Nucleotidesequences
PCR mutagenesis primers
pMM3 SfaSI For 5′-CCAAAGTCATGCGCGATCGCCGACAAAACACATACTTG-3′
pMM3 SfaSI Rev 5′-CAAGTATGTGTTTTGTCGGCGATCGCGCATGACTTTGG-3′
VHH primers
VHH BbsI NcoI For 5′-TTTTTTGAAGACGCCATGGCCCAGGTAAAGCTGGAGG-3′
VHH SfaSI Rev 5′-TTTTTTGCGATCGCTGAGGAGACGGTGACCTGGGTCC-3′
VHH Fc primers
VHH Fc BbsI SS For 5′-TTTGAAGACAAAGGTATGGCTAAAACAAATCTCTTTTTA-3′
VHH Fc BbsI HK Rev 5′-TTTGAAGACAAAAGCTCATTATAATTCATCCTTGTGATG-3′