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. 2013 Jul 22;8(7):e70554. doi: 10.1371/journal.pone.0070554

Figure 1. Eosinophils and PCs are found in close proximity in the human bone marrow.

Figure 1

BM biopsies were obtained from normal donors (A, C) and MM patients (B, D) and stained with H&E (A, B) or using immunofluorescence (C, D) for selective visualization of PCs and Eos. In immunofluorescence staining, PCs were stained with anti-CD138 mAb (green) and Eos were stained with chromotrope 2R (red). Autofluorescent red blood cells are shown in yellow in these overlaid images. Images are representative of 5 normal donor and 10 MM patient BM biopsies. (E) Quantitation of Eos across 6 random fields from each immunofluorescence-stained sample dividing Eos into 3 categories: 1) Eos in direct contact with PCs; 2) Eos within a 3-cell distance of PCs; and 3) Eos more than a 3-cell distance away from the closest PC. Samples #1-5 are BM biopsies from normal donors. Samples #6–8 are from patients with MGUS. Samples #10–12 are from patients with SMM. Samples #9 and 13–15 are from patients with MM. See Table S1 for more detail.