Figure 3. Eos enhance KAS-6/1, DP-6, and KP-6 proliferation in a contact-independent manner.

BM and PB Eos SN were collected as described in the Methods section. HMCL were cultured with or without BM (A) or PB (B) Eos SN in the presence or absence of IL-6. Proliferation of HMCLs was determined via [3H]TdR-incorporation. CM, control medium. (C) Proliferation of HMCLs either alone, in direct co-culture with BM Eos, or in the presence of BM Eos across a 0.4 µm-transwell was assessed. Results are representative of 3 independent experiments. * p<0.05; ** p<0.001; n.s., not significant.