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. 2013 Jul 22;8(7):e70554. doi: 10.1371/journal.pone.0070554

Figure 5. BM Eos and stromal cells (SC) have non-redundant roles in their support of MM cell proliferation.

Figure 5

(A) DP-6 cells were cultured either alone, in the presence of BM Eos, partially irradiated HS-5, or both. Cells were cultured at a ratio of 1∶1∶0.4 (DP-6:Eos:HS-5). Proliferation of DP-6 was assessed using a [3H]TdR-incorporation assay. (B) Varying numbers of BM Eos and HS-5 cells were co-cultured with 50,000 DP-6 cells and the proliferation of these cells was evaluated using [3H]TdR-incorporation. (C) Proliferation of KAS-6/1 (left) and KP-6 (right) was assessed after treatment with culture SN from HS-5, Eos, or HS-5 and Eos co-cultures. Additionally, HS-5 SN and Eos SN were mixed at a 1∶1 ratio and used to treat HMCLs. Results are representative of 3 independent experiments. * p<0.05.