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. 2013 Jul 22;8(7):e69419. doi: 10.1371/journal.pone.0069419

Figure 10. TcPRODH activities in mitochondrial vesicles (Mc) and total extracts from epimastigote forms (ET).

Figure 10

(A) Western blot analysis of ASATm (mitochondrial isoform of aspartate aminotransferase) (left panel) and TcPRODH (right panel) expression. ASATm (mitochondrial isoform of aspartate aminotransferase), an enzyme localized in the mitochondrial matrix is absent from mitochondrial vesicles. (B) Activities of citrate syntase, cs (matrix marker) and fumarate reductase, FMR (mitochondrial membrane marker). (C) Activity of TcPRODH in total extracts and mitochondrial vesicles of T. cruzi epimastigotes. These activities indicate that vesicles lack matrix content. (D) Reduction of cytochrome c in T. cruzi epimastigote mitochondrial vesicles in the presence L-proline, with or without antimycin A.