TABLE 2.

Results of protease effects on key endogenous physiological proteins/peptides using a designated protease blend14–16

KEY PROTEINS	MODULATORY EFFECTS*
TNF-α	Rapid reduction in monomeric and trimeric forms
IL-6/IL-6R	Sustained reduction
Substance P (neuropeptide)	Rapid reduction
Metalloproteinases (MMPs)	Varies degrees of reduction in several MMPs including MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, and MMP-9. Some MMPs with rapid reduction (i.e., MMP-1, MMP-9). Some MMPs with no reduction (i.e., MMP-10, MMP-13)
IL-1 (IL-1 alpha, IL-1 beta)	No reduction
PDGF	No reduction

^*Note that the magnitude and time course of reduction (degradation) of a given protein in these assays does not necessarily correlate with the magnitude/course of clinical effect and does not imply complete elimination of in vivo protein activity; clinical studies are needed to capture effects in patients with various skin conditions (i.e., normal skin, xerosis, atopic skin/eczematous disorders, psoriasis, rosacea, corticosteroid- treated skin, geriatric skin, etc.)

TNF=tumor necrosis factor; IL=interleukin; R=receptor; PDGF=platelet-derived growth factor