Figure 11. Agglutinative activity of control white shrimp (A), shrimp that received heat-killed Vibrio alginolyticus (HVa) (B), and shrimp that received formalin-inactivated V. alginolyticus (FVa) (C) after 7 days, and then were subjected to live V. alginolyticus (LVa).

Shrimp that received FVa and HVa showed higher agglutinative activities. Agglutinated “bacteria cluster” phagocytized by hyaline cells (HC) (D) and semi-granular cell (SGC) (E) were observed in both 7-day-HVa-PE shrimp and 7-day-FVa-PE shrimp, by both HC (F) and SGC (G) in 14-day-HVa-PE shrimp and 14-day-PE shrimp, and by both HC (H) and SGC (I) in 21-day-Hva-PE shrimp and 21-day-FVa-PE shrimp, and then received SE. The intact of different V. alginolyticus including LVa (J), HVa (K), and FVa (L) were strained with Liu’s straining. Scale = 5 µm (A∼I), and 2 µm (J∼L).