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. 2013 Jul 22;8(7):e69133. doi: 10.1371/journal.pone.0069133

Figure 1. Recessive suppressors of Glc7 overexpression.

Figure 1

A) JC746-9D (wt), JFY183 (gac1), JC1287-1C (reg1), JC938-5C (glc8), and JC1583 (reg2) transformed with GAL1p-GLC7 plasmids, p2562 or pKC978 (even rows) or control plasmids pRS315 or pRS316 (odd rows) were grown in selective raffinose medium and then serial five-fold dilutions were spotted on –Ura or –Leu glucose, YEP-galactose, or –Ura or –Leu galactose plates. B) Phospho-Glc8 is required for Glc7 overexpression lethality. JC938-5C (glc8) transformed with pYT251 (GAL1p-GLC7) and either p1945 (GLC8), pYT115 (GLC8-T118A), or p1614 (GAL1p-GLC8) were grown on –Trp –Ura galactose (Gal) or glucose (Glc). C) Diploids JC746, JC746/V76B8, JC746/RG200 and JC1378 transformed with pYT251 (GAL1p-GLC7) or pRS314 (control) were grown on –Trp galactose. The SDS22 genotypes of the host strains are shown. In all panels, the galactose medium induced GLC7 expression from the GAL1 promoter.