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. 2013 Jul 22;8(7):e69133. doi: 10.1371/journal.pone.0069133

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© 2013 Cannon, Ghosh

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

Crude extracts of SBY625/pRS426 (lanes 1-3), SBY625/p2509 (lanes 4–6), W303-1A (lane 7), SBY625 (GLC7-HA3) (lanes 8, 9), JC1552-17A (lane 10), JC1535 (lane 11), JC746-9D/YCp50 (lane 12), JC746-9D/YCp50-HA-GLC7 (lane 13), JC1338-20A/YCp50 (lane 14), and JC1338-20A/YCp50-HA-GLC7 (lane 15) were separated by SDS-PAGE, blotted and probed with anti-HA or anti-Pgk1 antibodies. All lanes except lane 9 have 20 µg protein,which has 10 µg. All cultures except those in lanes 7–11 were grown in minimal medium; those in lanes 7–11 were grown in YEP-glucose. The pRS426 and p2509 transformants (lanes 1–6) were induced with the indicated final concentration of CuSO₄ during the last two hours of growth. The Glc7/Pgk1 ratio was calculated from film densitometry.