Figure 4. Dominant suppressors of ipl1.
A) Suppression of ipl1 by several high-copy genes. JC1126-15B (ipl1-1) transformed with plasmids (pRS426, p2665, p2509, p2510, p2615, and p2613 respectively) with the indicated genotypes were incubated on –Ura plates at the indicated temperatures. Each spot had approximately 105 cells. B) SDS22-RM45 is not a dominant suppressor of ipl1. Homozygous ipl1 diploid strains, JC1630 (SDS22/SDS22) and JC1631 (SDS22/SDS22Δ) transformed with pRS316 (control), pAG108 (SDS22), or pAG-RM45 (SDS22-RM45) were incubated on –Ura plates at the indicated temperatures. C) Suppression of ipl1 by high-copy SDS22-S56am. JC1126-15B transformed with pAG108 (SDS22) or p2665 (SDS22-S56am) were incubated on –Ura plates at the indicated temperatures. Fivefold serial dilutions were spotted and grown for three days in panels B and C.