Fig. 1.

Model thiol SPR system. A. Typical membrane phospholipids, except for cardiolipin. Cardiolipin is most often found in the mitochondria of cells, however, NAbs have been shown to bind the lamellar and reversed hexagonal phases of cardiolipin. For simplicity, the acyl chain for all lipids is shown saturated with an abbreviated chain length. B. ω-substituted alkane thiols with different chemical functionality used to mimic membrane phospholipids. At pH 7.4 thiol monolayers were negatively charged (MUA), positively charged (AUT), polar (MUD), and nonpolar (ODT). C. Schematic of a model 11-mercaptoundecanoic acid (MUA) surface on a gold coated SPR sensor chip.

** Fig. 1 intended for color reproduction on the Web and in print