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. 2013 Jul 22;202(2):231–240. doi: 10.1083/jcb.201301090

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© 2013 Atkins et al.

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Figure 5. — Ste20 and Iqg1 mediate the inhibitory effect of active Cdc42 on cytokinesis and cell separation. (A) Cells transformed with 2μ IQG1 or a control vector were synchronized as in Fig. 3 E, and the percentage of large-budded or connected cells was determined 50 min after release. Graph shows means ± SEM from three experiments using independent 2μ-containing isolates (B) Cells were synchronized and scored as in A. Graph shows means ± SEM for three experiments. Bar, 10 µm. (C) Percentage of cells with contracting Iqg1-GFP dot 20 min after release from CDC20 arrest. Data are representative of two experiments; n > 200 cells. WT, wild type.