Figure 2.

Spontaneous tumorigenesis in K243R/+ mice. (A) Tumor incidence was assessed using Kaplan-Meier graphs. The tumor-free survival analysis (left) includes both benign and malignant tumors; the cancer-free survival analysis (right) includes only the malignant cancers. The statistical analyses were performed using SPSS software. Total number of mice analyzed cumulatively: WT, n = 41; K243R/+, n = 121. (B) Representative H&E-stained sections of the major pathologies found in the K243R/+ mice. The insets show high-magnification images. (C) H&E staining of spleens. (D–F) Analysis of lymph node cells. (D) Freshly isolated lymph node cells were immunostained using the indicated antibodies and subjected to flow cytometric analysis. (E) Lymph node sections from the K243R/+ mice were stained using an anti-B220 antibody and were counterstained with hematoxylin. (F) Wright-Giemsa staining of cytocentrifuged lymph node cells. (G) Megakaryocytic leukemia in the K243R/+ mice. The spleens were stained with H&E (left) or anti–von Willebrand factor (vWF) antibody and hematoxylin (right).