Figure 5.

RNAi induced by dsPns11 inhibited the formation of the tubules without significant effects on RGDV multiplication in VCMs. Twenty-four hours after transfection with dsGFP (A) or dsPns11 (B), VCMs were inoculated with RGDV at an MOI of 10 and cultured in the presence of virus-neutralizing antibodies. At 72 hpi, cells were immunolabeled with Pns11-FITC and P8-rhodamine, then examined with confocal microscopy. Images are representative of multiple experiments with multiple preparations. Bars, 30μm. (C) RNAi induced by dsPns11 significantly reduced the expression of Pns11 but not P8 of RGDV in virus-infected VCMs as shown here in Western blots. Protein extracts from cells transfected with Cellfectin, dsGFP or dsPns11 were separated by SDS-PAGE to detect Pns11 or P8 with Pns11-specific or P8-specific antibodies, respectively. Insect actin was detected with actin-specific antibodies as a control.