Figure 2.

Internally Stalled Ribosome-Nascent Chains Are Ubiquitinated

(A) Line diagrams of transcripts coding for truncated β-VHP, truncated β-VHP with a poly(A) tail (β-VHP-pA), and full-length β-VHP with a polybasic tract (β-VHP-K12).

(B) Truncated β-VHP with or without a poly(A) tail was translated and subject to RNase treatment. The migration of nascent chain-tRNA (NC-tRNA) and nascent chain (NC) is indicated.

(C) Analysis of β-VHP-pA as in Figure 1C.

(D) β-VHP-K12 was translated and subject to RNase treatment or immunoprecipitations with antibodies against either the N or C terminus of the protein. Immunoprecipitations of β-VHP-FL translations are shown for comparison.

(E) Analysis of β-VHP-K12 as in Figure 1C. See also Figure S2.