Skip to main content
. 2013 Jul 2;139(4):447–458. doi: 10.1111/imm.12092

Figure 7.

Figure 7

Increased oxidative stress in Ts65Dn haematopoietic progenitors. (a) Intracellular glutathione levels were measured in the indicated thymocyte sub-populations by flow cytometry using monochlorobimane (MCB) as described in the Materials and methods in euploid (open bars) or Ts65Dn (closed bars) mice (n = 4,*P < 0·05 by paired t-test). (b) DCFDA oxidation in the indicated thymic subsets was measured as described in the Materials and methods in cells from euploid (open bars) or Ts65Dn (closed bars) mice. The data are expressed as the per cent increased DCFDA fluorescence in Ts65Dn cells over euploid cells ± SEM (n = 4,*P < 0·05 by paired t-test). (c) NQO1 mRNA expression was assessed in whole thymus (c) and Lin− bone marrow (d) by quantitative PCR. These data are presented as the expression of the indicated mRNA relative to control HPRT mRNA (n = 5, *P < 0·05, **P < 0·01 by paired t-test).