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. 2013 Jul 2;139(4):447–458. doi: 10.1111/imm.12092

Figure 8.

Figure 8

Notch activation and microRNA (miRNA) expression in Ts65Dn thymuses and bone marrow (BM). (a,b) Hes-1 mRNA expression was assessed in (a) total thymus or (b) lineage negative (Lin−) BM cells by quantitative PCR in Ts65Dn (closed bar) and euploid controls (open bar). These data are expressed as the normalized expression of the indicated mRNA relative to control HPRT mRNA (n = 5, *P < 0·05 by paired t-test). (c,d) miR125 and miR155 expression were assessed in (c) total thymus and double-negative (DN) thymocytes or (d) Lin− and total BM cells by quantitative PCR as described in the Materials and methods. These data are presented as the normalized expression of the indicated miRNA relative to control u6 rRNA (n = 5,*P < 0·05 by paired t-test).