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. 2013 Jul 2;139(4):472–483. doi: 10.1111/imm.12094

Figure 4.

Figure 4

The capacity of equine monocyte-derived dendritic cells (MoDC) to take up antigen. (a) Effect of stimulation and activation on equine dendritic cells endocytic capacity. Mo, immature MoDC (iMoDC) and mature MoDC (mMoDC) were incubated with 20 μg/ml of ovalbumin (OVA) at 4° and 37° for 1 hr. The percentage of cells expressing allophycocyanin-conjugated OVA (OVA-APC) represent the difference in values obtained between the 37° and 4° (control). Monocytes have the greatest ability to take up OVA compared with DC while iMoDC are better at uptake than mMoDC. (b) Effect of stimulation and activation on equine DC phagocytic capacity. Immature MoDC and mMoDC wrere incubated at 4° and 37° for 1 hr with an FITC-conjugated microsphere,1·0 μm in size, at a 5 : 1 (beads/cell) ratio. Immature MoDC compared with mMoDC have a higher phagocytic activity. There was no significant difference between Mo and iMoDC. The percentage of positive cells represents the average mean ± SEM (n = 3). For single comparisons between Mo→iMoDC and iDC→mMoDC, a two-tailed paired Student's t-test was used. * indicate significant differences between sample means P < 0·05.