Epstein–Barr virus (EBV)-specific T-cell responses determined by standard and cultured ELISPOT assays in solid organ transplant recipients. Peripheral blood mononuclear cells (PBMC) from three heart transplant recipients (a–c) and a lung transplant recipient (d) were evaluated by standard and/or cultured ELISPOT assays in response to peptide pools (15 amino acids in length with an 11-amino-acid overlap) representing the full-length lytic (BZLF1 and BMRF1) and latent (EBNA1, EBNA3a, EBNA3b, EBNA3c, LMP1 and LMP2) EBV proteins. Results are shown as net spots/million PBMC. Error bars represent the SD of the mean. Blood was obtained at month 6·1 (patient P01), 9·7 (patient P02), 6·7 (patient P03) and 62·8 (patient P04), respectively, after transplantation. T-cell subsets indicated are at the time of ELISPOT analyses. nd, not done.