. 2013 Jul 23;3:2264. doi: 10.1038/srep02264

Table 3. List of plasmid constructs used in this study.

Plasmid construct	Description	Reference or source
pProEx-HTc	E. coli expression vector with N-terminal His₆-tag	Invitrogen
pProEx-HTc-SahH	Expression of His₆-SahH in E. coli	This study
pProEx-HTc-PknB_c	Expression of His₆-PknB_c (cytosolic domain) in E. coli	40
pProEx-HTc-PstP_c	Expression of His₆-PstP_c (cytosolic domain) in E. coli	40
pETDuet-1	E.coli dual expression vector containing His₆-tag in MCS-1 and S-tag in MCS-2	Novagen
pETDuet-SahH	Expression of His₆-SahH in MCS-1 in E. coli	This study
pETDuet-PknB	Expression of MBP-PknB in MCS-2 in E. coli	27,39
pSD5	Mycobacterial expression vector with Kanamycin resistance	37
pSD5-SahH	Expression of His₆-SahH in M. smegmatis MC² 155	This study
pYUBDuet	Inducible shuttle vector for expression of proteins in M. smegmatis MC² 4517	38
pYUBDuet-SahH	Expression of SahH under IPTG-inducible promoter in M. smegmatis MC² 4517	This study