Table II.

Residues whose Chemical Shifts Undergone Perturbations in Presence of Organic Solvents

Solvents	Residues faced perturbation in chemical shift
Acetone	G11, G13, K44, G46, L108, V136, H156
Acetonitrile	G11, G13, D43, K44, G46, N48, F58, L108, V136, G153, H156
DMF	G11, G13, G46, Y49, K61, L63, Y89, L108, V136, H156,
DMSO	G13, K44, G46, N48, Y49, E65, Y89, L108, T109, V136, H156
Isopropanol	G11, G13, K44, G46, V62, A105, L108, V136, G153, H156, G158
Methanol	I12, G13, S15, K44, G46, V62, N82, L108, T109, V136, V149, H152, H156, Y161
Active site residuesa	I12, A15, F17, N18, S77, M78, A105, L108, D133, M134, I135, L140, G155, H156, I157, L160, and Y161

^aIdentity of active site residues is taken from wild type B. subtilis lipase.²²