Figure 2.

The PfaC sequence was analyzed using the UMA algorithm to detect the presence of additional structured domains within the DH region. The areas of high UMA score are typically considered to coincide with structured domains. The data shows that in addition to the four known domains detected by sequence alone (shown on bar above the graph), there are two previously uncharacterized regions of high UMA score directly N-terminal to the DH domains. These two new regions of predicted structure were termed DH′ pseudo-domains. This exercise in primary sequence analysis resulted in the design of DH1-DH2-UMA, a tretradomain fragment with activity.