Figure 5.

Design of soluble and active DH constructs. (A) A number of protein constructs were designed to contain single and multiple DH domains. Of the fragments that were cloned, only the DH1-DH2-UMA, which was made according to the UMA results, was found to be soluble and stable. (B) SDS-PAGE gel shows that DH1-DH2-UMA protein can be made with a high yield (1 mg pure protein per L of culture) and purity. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]