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. 2013 May 20;22(7):954–963. doi: 10.1002/pro.2278

Table II.

Oligonucleotides Used to Amplify the Different DH Fragments in This Study

DH construct Oligonucleotide sequence
DH1S Fwd: 5′- CATGCATGGGATCCAACTTGCTAGACGCAAATATCGCA -3′
Rv: 5′- CATGCATGCCCGGGTCATGATTCTTCTTTGATCATCACG -3′
DH1L Fwd: 5′- CACCTTCTCTTACGAATGTTTCGTTGGC -3′
Rv: 5′- CATGCATGCCCGGGTCATGATTCTTCTTTGATCATCACG -3′
DH2S Fwd: 5′- CATGCATGGGATCCAACTTACTGGATAAGAAAGCCGTT -3′
Rv: 5′- TCAGGCTTCTTCAATACAGATTGC -3′
DH2L Fwd: 5′- CATGCATGGGATCCTTCAGCTTCGAACTCAGTACCGA -3′
Rv: 5′- TCAGGCTTCTTCAATACAGATTGC -3′
DH1-DH2-S Fwd: 5′-CACCAACTTGCTAGACGCAAATATCGCA-3′
Rv: 5′-TCAGGCTTCTTCAATACAGATTGC-3′
DH1-DH2-L Fwd: 5′-CACCTTCTCTTACGAATGTTTCGTTGGC-3′
Rv: 5′-TCAGGCTTCTTCAATACAGATTGC-3′
DH1-DH2 UMA Fwd: 5′-CACCCGCAAACCTTGTATCTGGGATTA-3′
Rv: 5′-TCAGGCTTCTTCAATACAGATTGC-3′
DH1(H70A) Fwd: 5′-ACTTCCCTTGTGCATTCAAAGATGA -3′
Rv: 5′- TCATCTTTGAATGCACAAGGGAAGT-3′
DH2(H70A) Fwd: 5′- CTTCCAATTCGCATTCCACCAAG-3′
Rv: 5′-CTTGGTGGAATGCGAATTGGAAG-3′