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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Gastroenterology. 2013 Apr 2;145(1):138–148. doi: 10.1053/j.gastro.2013.03.048

Figure 5. Enzymatic activity of wild-type PC1/3 and novel PC1/3 variants.

Figure 5

The enzymatic activities of secreted PC1/3 proteins in conditioned medium were assayed using a fluorogenic assay. Four replicates per transfection condition were assayed in triplicate, and maximum rates were normalized to WT PC1/3. Maximum activity rates are shown as the mean ±S.D., n=4 wells. Data represent one of 3 independent experiments. Bars represent mean ± standard deviation, and unpaired Student’s t-test was used to assess difference between mutant and WT activities with *:P-value <0.001, **:P-value <0.05. All experiments were independently repeated in triplicate wells at least three times, with similar results.