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. 2013 Jul 23;3:187. doi: 10.3389/fonc.2013.00187

Figure 2.

Figure 2

Human Eg5 rescues depletion of the Drosophila kinesin-5 family member Klp61F. (A) Western blot analysis of cell lysates prepared from induced and uninduced; control and Klp61F RNAi-treated cells blotted with anti-Eg5 to monitor induction of Eg5-mCherry, anti-Klp61F to confirm depletion of the motor and anti-α-tubulin as a loading control. Cells depleted of Klp61F and with Eg5-mCherry induced are referred to as RepEg5 cells. (B) Quantification of the percentage of cells with monopolar spindles in control RNAi cells versus Klp61F-depleted Eg5-mCherry induced and uninduced cells. (C) An example of a field of view containing a RepEg5 cell with a bipolar spindle next to a Klp61F-depleted cell that is not expressing Eg5-mCherry and has a monopolar spindle. In the merged image GFP-α-tubulin is shown in green, Eg5-mCherry in red, and DAPI-stained DNA in blue. Error bars are standard error of the mean (SEM). Scale bar is 10 μm.