Figure 4.

Efficient collapse of bipolar spindles into monopoles in STLC-treated RepEg5 cells requires the minus end-directed Drosophila kinesin-14 motor Ncd. (A) Representative maximum projection images of spindle morphologies in control RNAi- and Ncd RNAi-treated RepEg5 cells in either DMSO or 1 μM STLC. In the merged images GFP-α-tubulin is green, Eg5-mCherry is red, and DAPI-stained DNA is blue. (B) Quantification of spindle structures in RepEg5 cells treated with 1 μM STLC in the presence and absence of Ncd (n = 200 spindles in control RNAi; n = 206 structures in Ncd RNAi). Error bars are SEM. Scale bars are 10 μm.