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. 2013 Jul 23;3:187. doi: 10.3389/fonc.2013.00187

Figure 5.

Figure 5

Inhibition of Eg5 with STLC is reversible in RepEg5 cells. (A) Still frames from a time-lapse of a RepEg5 cell before and after the addition of 1 μM STLC and following STLC wash out. STLC was added between 0:00 and 2:00 and washed out at 21:44. Addition of STLC causes the RepEg5 bipole to collapse into a monopole within 8 min. The RepEg5 monopole reorganizes into a bipole over ∼40 min following STLC wash out and undergoes a bipolar anaphase ∼54 min after removal of the drug. GFP-α-tubulin is shown in green and Eg5-mCherry is shown in red. (B) Quantification of spindle morphologies over time (in minutes) after STLC wash out (t = 0, n = 503 spindles; t = 30, n = 305; t = 60, n = 403; t = 120, n = 199). The percentage of monopoles decreases and the percentage of bipoles increases over time following removal of STLC. Error bars are SEM. Scale bar is 10 μm.