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. 2012 Jul-Sep;13(3):151–157.

Table 1.

Primers used in RT-PCR for adipocyte markers

Gene Sequence Annealing temperature (°C) Length (bp) Gene bank code
PPARα
F 5’–CCGCCTCCTTCGGCGTTC–3’ 58 149 NM_001001928.2
R 5’–AGCTCCAAGCTACTGTGGTGACA–3’
β- actin
F 5’–CGTGACATTAAGGAGAAG–3’ 56 202 NM_001101
R 5’–TGATGGAGTTGAAGGTAF–3’