Figure 3.
Effect of androstenedione on Cx43 expression. A. Western blot analysis of Cx43 protein in normal rat ovaries (lane a) and in androstenedione treated rats’ PCO ovaries (lane b). Lane H: heart sample Cx43 control. B. Western blot analysis of Cx43 protein in GCs on day 4 of culture. Cx43 proteins expressed in GCs cultured on EHS-drip (lane a), in the presence of 10−7 M (lane b) or 10−5 M (lane c) androstenedione. P0, unphosphorylated form of Cx43 protein, P1 phosphorylated active form of Cx43. β-actin protein shows for equal loading