Fig. 2.

Selective observations of intramolecular NOE cross peaks within the inhibitor bound to Grb2 SH2. (a) 2D NOESY spectrum recorded using a complex of the inhibitor and ¹⁵N-labeled Grb2 SH2 in a ²H₂O solution, where intramolecular cross peaks derived from both the inhibitor and the protein and intermolecular cross peaks between the inhibitor and the protein were observed simultaneously. (b) 2D NOESY spectrum recorded using a complex of the inhibitor and ²H/¹⁵N-labeled Grb2 SH2 domain in a ²H₂O solution. Cross peaks derived from the inhibitor were selectively observed