Fig. 4.

(a) Strip plots of ¹H-¹H planes extracted from a 3D ¹³C-filtered NOESY spectrum recorded using the complex of the inhibitor and ¹³C/¹⁵N-labeled Grb2 SH2 domain in a 90% H₂O solution. Signals at 4.75 ppm on the F3-axis arised from the solvent. The resonance assignments and corresponding ¹H and ¹³C chemical shifts of Grb2 SH2 domain were given in each strip. Intermolecular NOE correlations between the inhibitor and Grb2 SH2 domain were annotated. (b) Strip plots of ¹H-¹H planes extracted from 3D ¹⁵N-edited NOESY recorded using a complex of the inhibitor and the ²H/¹⁵N-labeled Grb2 SH2 domain in a 90% H₂O solution. Intermolecular NOE correlations between amide protons of Grb2 SH2 and the inhibitor were labeled. Note that the intramolecular NOE correlations of amide-amide pairs of Grb2 SH2 domain were also observed. Signals at 4.75 ppm on the F1-axis arised from the solvent. Asterisk showed the artifact noise