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. Author manuscript; available in PMC: 2013 Jul 23.
Published in final edited form as: Rev Mex Cienc Farm. 2011 Oct-Dec;42(4):57–65.

Figure 1.

Figure 1

A) Relative hPHT1 mRNA expression levels in COS-7/Mock and COS-7/hPHT1 stable transfects (COS-7/hPHT1 normalized to COS-7/Mock) at the passage number 7 immediately prior to conducting uptake studies. B) Relative hPHT1 mRNA expression levels in COS/Mock and COS-7/hPHT1 stable transfects (COS-7/hPHT1 normalized to COS-7/Mock) at final passage number 15 after completion of uptake experiments. Relative PHT1 mRNA expression was determined by qRT-PCR, using gene specific primers, under optimized reaction conditions as described in the methodology section. Results are plotted as ΔCt normalized to the GAPDH housekeeping gene. [11]