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Concentration-dependent [3H] histidine uptake in stably transfected COS-7/Mock (A) and COS-7/hPHT1 (B) with the extrapolated active/passive uptake components determined at 5 mM in both cell lines. The active (saturable) uptake of each substrate is calculated by subtracting the calculated passive (non-saturable) uptake values from the total uptake per equation (1).
