Table 1.
Table showing the Michaelis-Menten parameters (VMAX and KM) extrapolated and calculated from the [3H] Histidine concentration dependent curve illustrated in Figure 4 and extrapolated from the curves appearing in Figure 5 panels A) and B) for the Mock and hPHT1 stably transfected cells, respectively.
Substrate | VMAX (nmol/mg protein) | KM (mM) | Kd (mM) | |||
---|---|---|---|---|---|---|
Histidine | MOCK | PHT1 | MOCK | PHT1 | MOCK | PHT1 |
37.5 ± 5.78 | 34.0 ± 2.60 | 0.203 ± 0.066 | 0.160 ± 0.027 | 4.64 ± 2.14 | 7.25 ± 1.03 |