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. Author manuscript; available in PMC: 2013 Jul 23.
Published in final edited form as: J Biomed Mater Res A. 2011 Jul 25;99(1):47–57. doi: 10.1002/jbm.a.33162

Figure 4.

Figure 4

Swelling of beads consisting of alginate, Alg-N3, and Alg-N3 stabilized with crosslinkers as a function of pre-incubation time, solvent, and time. A). Bead swelling following three 45 min washes with dH2O as a function of pre-incubation time; B) Bead swelling following EDTA chelation as a function of pre-incubation time; and C) Bead swelling over 14 days when incubated in DMEM. X indicates inability to fabricate beads due to high solution viscosity. ¥ indicates bead dissolution following EDTA incubation. Error bars represent standard deviations with n of 10 beads. * indicates statistical difference between no pre-incubation time for same polymer combination. D) Microscopic images of (I) Ba2+-cross-linked alginate bead in MOPS buffer and (II) Alg-N3+4 bead without Ba2+ in MOPS after being compressed gently using two pipette tips.