FIG. 5.

Lentiviral vector treatments restore the RyR1 calcium release activity in differentiated MF cells. Calcium imaging performed on control CF cells (empty square), untreated MF cells (black square), and MF cells treated with U7-Crtl vector (black triangle) or with U7-D+E vector (gray square), and differentiated for 8–10 days before calcium imaging. (a) Fluorescence variation curves induced by application during 50 sec (black bar) of CmC (500 μM) in the presence of 2 mM external calcium, presented as mean (symbols)±SEM (dotted lines). The maximal amplitude of the peak for each curve is presented in the bar plots on the right, with the number of myotubes analyzed in each bar. (b) Fluorescence variation curves induced by membrane depolarization (KCl 140 mM) applied during 50 sec (black bar) in the presence of 2 mM external calcium, presented as mean (symbols)±SEM (dotted lines). ****p<0.0001, ANOVA analysis followed by Bonferroni multiple comparison test. CmC, 4-Chloro-m-Cresol.