Table 2.
Genes up- or downregulated in S. mutans UA159 following nisin A or nukacin ISK-1 treatment
| Treatment, regulation change, and gene IDa | Gene name | Regulation by TCS | Fold differenceb | Gene function |
|---|---|---|---|---|
| Nisin A | ||||
| Upregulated genes | ||||
| SMU.655 | mutE | +c | 17.3 | ABC transporter, permease |
| SMU.1961 | levD | − | 17.1 | Fructose-specific enzyme IIA component |
| SMU.654 | mutF | + | 16.8 | ABC transporter, ATP binding |
| SMU.656 | mutE2 | + | 14.8 | ABC transporter, ATP binding |
| SMU.657 | mutG | + | 14.6 | ABC transporter, permease |
| SMU.658 | nsrX | + | 12.9 | Conserved hypothetical protein |
| SMU.1958 | levF | − | 10.8 | Fructose-specific enzyme IIC component |
| SMU.659 | nsrR | + | 10.2 | Response regulator |
| SMU.1957 | levG | − | 9.0 | Fructose-specific enzyme IID component |
| SMU.1960 | levE | − | 7.6 | Fructose-specific enzyme IIB component |
| SMU.1956 | − | 7.2 | Conserved hypothetical protein | |
| SMU.660 | nsrS | + | 6.3 | Histidine kinase |
| SMU.1195 | − | 4.0 | ABC transporter permease protein | |
| SMU.1193 | yhcF | − | 3.9 | Transcriptional regulator, GntR family |
| SMU.1194 | yurY | − | 3.9 | ABC transporter, ATP binding |
| SMU.862 | − | 3.5 | Conserved hypothetical protein | |
| Nukacin ISK-1 | ||||
| Upregulated genes | ||||
| SMU.1148 | lctF | +d | 74.4 | ABC transporter, ATP binding |
| SMU.1149 | lctE | + | 67.3 | ABC transporter, permease |
| SMU.1150 | lctG | + | 53.3 | ABC transporter, permease |
| SMU.1705 | − | 6.5 | Conserved hypothetical protein | |
| SMU.1706 | − | 5.7 | Conserved hypothetical protein | |
| SMU.1704 | − | 5.2 | Conserved hypothetical protein | |
| SMU.1147 | lcrX | + | 4.7 | Hypothetical protein |
| SMU.1146 | lcrR | + | 4.4 | Response regulator |
| SMU.1145 | lcrS | + | 4.2 | Histidine kinase |
| SMU.753 | − | 4.1 | Conserved hypothetical protein | |
| Downregulated genes | ||||
| SMU.1960 | levE | − | 0.5 | Fructose-specific enzyme IIB component |
| SMU.1961 | levD | − | 0.3 | Fructose-specific enzyme IIA component |
| SMU.1877 | manL | − | 0.3 | Mannose PTS component IIAB |
| SMU.1878 | manM | − | 0.3 | Mannose PTS component IIC |
| SMU.1879 | manN | − | 0.3 | Mannose PTS component IID |
a
Gene identification (ID) numbers are from the GEO database of NCBI (http://www.ncbi.nlm.nhi.gov/geo/).
b
The fold change between bacteriocin-treated cells versus nontreated cells.
c
Regulated by NsrRS.
d
Regulated by LcrRS.